Inmetro  >
Metrologia >
Metrologia Aplicada à Biologia >
DIMAV | Artigos publicados em periódicos internacionais >

Por favor, utilize esse identificador para citar este item ou usar como link:

Título: New insights into the morphology of Trypanosoma cruzi reservosome
Autores: Sant’Anna, Celso
Pereira, Miria Gomes
Lemgruber, Leandro
Souza, Wanderley de
Silva, Narcisa Leal da Cunha e
Palavras-Chaves: Parasitologia
Biologia celular
Trypanosoma cruzi
Data: 2008
Citação: SANT'ANNA, Celso et al. New insights into the morphology of Trypanosoma cruzi reservosome. Microscopy Research and Technique, v. 71, p. 599-605, 2008.
Resumo: Reservosomes are late endosomes present only in members of the Schizotrypanum subgenus of the Trypanosoma genus and are defined as the site of storage of endocytosed macromolecules and lysosomal enzymes. They have been extensively described in Trypanosoma cruzi epimastigote: are bounded by a membrane unit, present an electron-dense protein matrix with electron-lucent lipid inclusions, being devoid of inner membranes. Here we performed a detailed ultrastructural analysis of these organelles using a variety of electron microscopy techniques, including ultrathin sectioning, uranyl acetate stained preparations, and freeze fracture, either in intact epimastigotes or in isolated reservosomes. New informations were obtained. First, both isolated and in situ reservosomes presented small profiles of inner membranes that are morphologically similar to the membrane surrounding the organelle. In uranyl acetate stained preparations, internal membrane profiles turned out to be longer than they appeared in ultrathin section images and traversed the organelle diameter. Internal vesicles were also found. Second, endocytosed cargo are not associated with internal vesicles and reach reservosomes on board of vesicles that fuse with the boundary membrane, delivering cargo directly into reservosome lumen. Third, electron-lucent bodies with saturated lipid core surrounded by a membrane monolayer and with unusual rectangular shape were also observed. Fourth, it was possible to demonstrate the presence of intramembranous particles on the E face of both internal vesicles and the surrounding membrane. Collectively, these results indicate that reservosomes have a complex internal structure, which may correlate with their multiple functions.
Descrição: 7 p. : il.
Tipo de documento: Artigo / Article
Unidade: Diretoria de Programa - Dipro
Aparece nas Coleções:DIMAV | Artigos publicados em periódicos internacionais

Arquivos neste Item:

Arquivo Descrição TamanhoFormato
SantAnna_2008.pdf499,11 kBAdobe PDFSob Embargo

Este item está licenciado com Licença Creative Commons
Creative Commons

Itens protegidos por copyright, com todos os direitos reservados, Salvo indicação em contrário.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Fale conosco