brasil.gov.br
DSpace

Inmetro  >
Metrologia >
Metrologia Aplicada à Biologia >
DIMAV | Artigos publicados em periódicos internacionais >

Por favor, utilize esse identificador para citar este item ou usar como link: http://hdl.handle.net/10926/1924

Título: Dynamics and 3D organization of secretory organelles of Toxoplasma gondii
Autores: Santos, Tatiana Christina Paredes
Souza, Wanderley de
Attias, Márcia
Palavras-Chaves: Toxoplasma gondii
Organela secretora
Reconstrução em 3D
Estereologia
Complexo apical
Data: 2012
Citação: SANTOS, Tatiana Christina Paredes; SOUZA, Wanderley de; ATTIAS, Márcia. Dynamics and 3D organization of secretory organelles of Toxoplasma gondii. Journal of Structural Biology, v. 177, p. 420–430, 2012.
Resumo: Micronemes, rhoptries and dense granules are secretory organelles of Toxoplasma gondii crucial for host cell invasion and formation of the parasitophorous vacuole (PV). We examined whether their relative volumes change during the intracellular cycle. Stereological analysis of random ultrathin sections taken at 5 min of interaction, 7 and 24 h post-infection demonstrated that the relative volume of each type of organelle decreases just after the respective peak of secretion. Micronemes are radially arranged below the polar ring, while rhoptries converge to but only a few reach the inside of the conoid. In contrast to the apical and polarized organelles, dense granules were found scattered throughout the cytoplasm, with no preferential location in the parasite cell body. Extensive observation of random sections indicated that each organelle probably secretes in a different region. Micronemes secrete just below the posterior ring and probably require that the conoid is extruded. The rhoptries passing through the conoid secrete at a porosome-like point at the most apical region. Dense granules secrete laterally, probably at fenestrations in the inner membrane complex. Immunocytochemistry showed that there are no subpopulations of rhoptries or dense granules, as a single organelle can contain more than one kind of its specific proteins. The vacuolar-like profiles observed at the apical portion of parasites just after invasion were confirmed to be empty rhoptries, as they were positively labeled for rhoptry proteins. These findings contribute for a better understanding of the essential behavior of secretory organelles.
Descrição: 11 p. : il., tab.
Tipo de documento: Artigo / Article
Unidade: DIPRO - Diretoria de Programa
Aparece nas Coleções:DIMAV | Artigos publicados em periódicos internacionais

Arquivos neste Item:

Arquivo Descrição TamanhoFormato
Santos_2012.pdf2,41 MBAdobe PDFSob Embargo
Estatísticas

Este item está licenciado com Licença Creative Commons
Creative Commons

Itens protegidos por copyright, com todos os direitos reservados, Salvo indicação em contrário.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Fale conosco